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A comprehensive analysis of fifteen genes of steviol glycosides biosynthesis pathway in Stevia rebaudiana (Bertoni)

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Title A comprehensive analysis of fifteen genes of steviol glycosides biosynthesis pathway
in Stevia rebaudiana (Bertoni)
 
Creator Kumar, Hitesh
Kaul, Kiran
Gupta, Suphla Bajpai
Kaul , V K
Kumar, Sanjay
 
Subject Plant sciences
Information and Science Management
 
Description Stevia [Stevia rebuaidana (Bertoni); family: Asteraceae] is known to yield diterpenoid steviol glycosides
(SGs), which are about 300 times sweeter than sugar. The present work analyzed the expression of various
genes of the SGs biosynthesis pathway in different organs of the plant in relation to the SGs content. Of the
various genes of the pathway, SrDXS, SrDXR, SrCPPS, SrKS, SrKO and three glucosyltransferases namely
SrUGT85C2, SrUGT74G1 and SrUGT76G1 were reported from stevia. Here, we report cloning of seven additional
full-length cDNA sequences namely, SrMCT, SrCMK, SrMDS, SrHDS, SrHDR, SrIDI and SrGGDPS followed by
expression analysis of all the fifteen genes vis-à-vis SGs content analysis. SGs content was highest in the
leaf at 3rd node position (node position with reference to the apical leaf as the first leaf) as compared to
the leaves at other node positions. Except for SrDXR and SrKO, gene expression was maximum in leaf at 1st
node and minimum in leaf at 5th node. The expression of SrKO was highest in leaf at 3rd node while in
case of SrDXR expression showed an increase up to 3rd leaf and decrease thereafter. SGs accumulated maximum
in leaf tissue followed by stem and root, and similar was the pattern of expression of all the fifteen
genes. The genes responded to the modulators of the terpenopids biosynthesis. Gibberellin (GA3) treatment
up-regulated the expression of SrMCT, SrCMK, SrMDS and SrUGT74G1, whereas methyl jasmonate and kinetin
treatment down-regulated the expression of all the fifteen genes of the pathway.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/934/1/Gene_reprint.pdf
Kumar, Hitesh and Kaul, Kiran and Gupta, Suphla Bajpai and Kaul , V K and Kumar, Sanjay (2012) A comprehensive analysis of fifteen genes of steviol glycosides biosynthesis pathway in Stevia rebaudiana (Bertoni). (Bertoni) Gene, 492. pp. 276-284.
 
Relation http://ihbt.csircentral.net/934/