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Comparative studies of volatile oil composition of Rhododendron anthopogon by hydrodistillation, supercritical carbon dioxide extraction and head space analysis

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Title Comparative studies of volatile oil composition of Rhododendron
anthopogon by hydrodistillation, supercritical carbon dioxide
extraction and head space analysis
 
Creator Guleria, S
Jaitak, V
Saini, R
Kaul , V K
Lal, Brij
G D, Kiran Babu
Singh , Bikram
Singh, R D
 
Subject Plant sciences
Natural Product Chemistry
 
Description Volatile oil composition of the leaves of Rhododendron anthopogon
(Ericaceae) growing wild in alpine Western Himalaya was studied using
different extraction techniques including SC-CO2 extraction and hydrodistillation
(HD). Results from different extraction methodologies were
compared with headspace analysis (HS) and evaluated for the effectiveness
of techniques in characterisation of various terpene categories and to assess
their influence on the yield and composition of volatiles. Variability in
constituents and in quantitative yields was observed. The results varied
with different extraction methods. A total of 27 constituents in SC-CO2
extraction, 31 in HD and 17 in HS analysis were identified. Constituents in
SC-CO2 and HD oils were identified by gas chromatography mass
spectrometry analysis. SC-CO2 extraction was carried out at 40 degree C and
140 bar pressure and the oil represented by major constituents as
�-caryophyllene (5.96%), �-humulene (4.06%) and p-menthadiene-2,9-
diol (7.28%); in HD, oil limonene (11.26%), �-caryophyllene (11.62%),
�-humulene (7.22%), and E-nerolidol (5.83%) dominated the oil and in HS
analysis, limonene (24.14%), �-terpinene (40.73%), �-terpinene (4.92%),
�-phellandrene (3.44%) and �-ocimene (7.15%) were present as major
constituents
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/943/1/31-01-2012_Comparative.pdf
Guleria, S and Jaitak, V and Saini, R and Kaul , V K and Lal, Brij and G D, Kiran Babu and Singh , Bikram and Singh, R D (2011) Comparative studies of volatile oil composition of Rhododendron anthopogon by hydrodistillation, supercritical carbon dioxide extraction and head space analysis. Natural Product Research, 25 (13). pp. 1271-1277.
 
Relation http://ihbt.csircentral.net/943/