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Induction of somatic embryogenesis and analysis of genetic fidelity of in vitro-derived plantlets of Bambusa nutans Wall., using AFLP markers

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Title Induction of somatic embryogenesis and analysis of genetic
fidelity of in vitro-derived plantlets of Bambusa nutans Wall.,
using AFLP markers
 
Creator Mehta, R.
Sharma, V
Sood, A
Sharma, Madhu
 
Subject Plant Biotechnology
Plant viruses
 
Description Abstract Bambusa nutans Wall., is an evergreen,
perennial, and multipurpose bamboo having strong culms,
which are largely used for construction, scaffolding, craft
purposes, pulp, and paper industry. Multiple shoots from
nodal segments (3–4 cm) of young branches of mature
culms were established in Murashige and Skoog (1962)
(MS) medium supplemented with various concentrations
of 6-benzylaminopurine (BAP) (1.0–6.0 mg l-1) or in
combination with a-naphthaleneacetic acid (NAA)
(0.5–1.0 mg l-1) or kinetin (Kn) (1.0–2.0 mg l-1).
February–March and December were found to be the best
seasons for culture establishments. Maximum shoots were
achieved on MS medium fortified with BAP (2.0 mg l-1).
Embryogenic callus (slightly greenish compact, globular,
and slow growing) was initiated from the base of severed
sprouted buds in 2–3 subsequent subcultures on MS medium
supplemented with 2,4-dichlorophenoxy acetic acid
(2,4-D) (5.0 mg l-1) under dark incubations. Maturation
and germination of well-organized somatic embryos was
achieved on MS medium containing BAP and 2,4-D
(1.0 mg l-1 each) with 20.0 mg l-1 ascorbic acid. Fullstrength
MS medium supplemented with 2% glucose
favored further development of proliferated somatic
embryos into plantlets. Genetic variations of fieldestablished
B. nutans plants regenerated through tissue
cultures were assessed by amplified fragment length
polymorphism (AFLP) analysis using 6 primer combinations.
Four hundred and seven scorable fragments were
amplified, of which 402 (98.8%) have recorded conservation
at various morphogenetic stages leading to plantlets
regeneration, therefore, revealed a high level of genetic
stability.
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/952/1/08-02-2012_Introduction.pdf
Mehta, R. and Sharma, V and Sood, A and Sharma, Madhu (2010) Induction of somatic embryogenesis and analysis of genetic fidelity of in vitro-derived plantlets of Bambusa nutans Wall., using AFLP markers. Journal of European Forest Research, 130 (5). pp. 729-736.
 
Relation http://ihbt.csircentral.net/952/