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Lipoxygenase in Caragana jubata responds to low temperature, abscisic acid, methyl jasmonate and salicylic acid

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Title Lipoxygenase in Caragana jubata responds to low temperature, abscisic acid, methyl
jasmonate and salicylic acid
 
Creator Bhardwaj, P K
Kaur, J
Sobit, R C
Ahuja, Paramvir Singh
Kumar, Sanjay
 
Subject Plant sciences
Natural Product Chemistry
 
Description Lipoxygenase (LOX) catalyses oxygenation of free polyunsaturated fatty acids into oxylipins, and is a critical
enzyme of the jasmonate signaling pathway. LOX has been shown to be associated with biotic and abiotic
stress responses in diverse plant species, though limited data is available with respect to low temperature and
the associated cues. Using rapid amplification of cDNA ends, a full-length cDNA (CjLOX) encoding
lipoxygenase was cloned from apical buds of Caragana jubata, a temperate plant species that grows under
extreme cold. The cDNA obtained was 2952 bp long consisting of an open reading frame of 2610 bp encoding
869 amino acids protein. Multiple alignment of the deduced amino acid sequence with those of other plants
demonstrated putative LH2/ PLAT domain, lipoxygenase iron binding catalytic domain and lipoxygenase_2
signature sequences. CjLOX exhibited up- and down-regulation of gene expression pattern in response to low
temperature (LT), abscisic acid (ABA), methyl jasmonate (MJ) and salicylic acid (SA). Among all the
treatments, a strong up-regulation was observed in response to MJ. Data suggests an important role of
jasmonate signaling pathway in response to LT in C. jubata.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/956/1/09-02-2012__Lipoxyhenase.pdf
Bhardwaj, P K and Kaur, J and Sobit, R C and Ahuja, Paramvir Singh and Kumar, Sanjay (2011) Lipoxygenase in Caragana jubata responds to low temperature, abscisic acid, methyl jasmonate and salicylic acid. Gene, 483. pp. 49-53.
 
Relation http://ihbt.csircentral.net/956/