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An improved method for thin layer chromatographic analysis of saponins

IR@CSIR-IHBT

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Title An improved method for thin layer chromatographic analysis of saponins
 
Creator Sharma, O P
Kumar, Neeraj
Bhat., Tej. K
Singh , Bikram
 
Subject Plant sciences
Natural Product Chemistry
 
Description a b s t r a c t
Analysis of saponins by thin layer chromatography (TLC) is reported. The solvent system was n-butanol:
water:acetic acid (84:14:7). Detection of saponins on the TLC plates after development and air-drying
was done by immersion in a suspension of sheep erythrocytes, followed by washing off the excess blood
on the plate surface. Saponins appeared as white spots against a pink background. The protocol provided
specific detection of saponins in the saponins enriched extracts from Aesculus indica (Wall. ex Camb.)
Hook.f., Lonicera japonica Thunb., Silene inflata Sm., Sapindus mukorossi Gaertn., Chlorophytum borivilianum
Santapau & Fernandes, Asparagus adscendens Roxb., Asparagus racemosus Willd., Agave americana L.,
Camellia sinensis [L.] O. Kuntze. The protocol is convenient, inexpensive, does not require any corrosive
chemicals and provides specific detection of saponins
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/963/1/Food_Chem._2011.pdf
Sharma, O P and Kumar, Neeraj and Bhat., Tej. K and Singh , Bikram (2012) An improved method for thin layer chromatographic analysis of saponins. Journal of Food Chemistry, 132. pp. 671-674.
 
Relation http://ihbt.csircentral.net/963/