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Intermolecular Interactions of Chrysanthemum virus B Coat Protein: Implications for Capsid Assembly

IR@CSIR-IHBT

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Title Intermolecular Interactions of Chrysanthemum virus B Coat
Protein: Implications for Capsid Assembly
 
Creator Singh, L
Hallan, Vipin
Zaidi , A A
 
Subject Plant sciences
Plant viruses
 
Description Chrysanthemum virus B coat protein constitutes
the viral capsid which, besides other functions,
encapsulates and protects the viral nucleic acid. We have
demonstrated homotypic interaction of the coat protein
subunits, essentially important for dimer formation, which
is the first step during capsid assembly in vivo and in vitro.
Interaction capacity of full length and truncated protein has
been investigated and important regions have been identified
through protein-protein interaction in yeast and by coimmunoprecipitation
assays. Complete coat protein was
found to interact strongly with similar subunits. Constructs
with 102 amino acids from the N-terminal and 64 amino
acids from C-terminal were found to be inconsequential for
dimer formation as they did not show any interaction with
similar subunits or with full length protein when analysed
for b-galactosidase or histidine prototrophy. Results suggest
that the region of 98–184 amino acids from the middle
plays an important role in the process, probably without the
involvement of N- and C- terminals.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/967/1/view.pdf
Singh, L and Hallan, Vipin and Zaidi , A A (2011) Intermolecular Interactions of Chrysanthemum virus B Coat Protein: Implications for Capsid Assembly. Indian Journal of Virol, 22 (2). pp. 111-116.
 
Relation http://ihbt.csircentral.net/967/