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DEVELOPMENT AND OPTIMIZATION OF AN HPLC METHOD FOR THE ROUTINE ANALYSIS OF CATECHINS, CAFFEINE, AND GALLIC ACID IN TEA (CAMELLIA SINENSIS)

IR@CSIR-IHBT

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Title DEVELOPMENT AND OPTIMIZATION OF AN HPLC METHOD FOR
THE ROUTINE ANALYSIS OF CATECHINS, CAFFEINE, AND GALLIC
ACID IN TEA (CAMELLIA SINENSIS)
 
Creator Sharma, Anupma
Bhondekar, Amol P
Bari, S S
Gulati, Ashu
Kapur, Pawan
Singla, M L
 
Subject Plant viruses
Microbiology
 
Description An efficient and rapid high performance liquid chromatographic (HPLC) assay was developed
for the quantification of catechins [(þ) – catechin, (�) – epigallocatechin, (�) – epigallocatechingallate,
(�) – epicatechin, and (�) – epicatechingallate], caffeine, and gallic acid in tea (Camellia
Sinesis var. sinesis). The assay was optimized by varying sample strength, column temperature,
gradient type, and detection wavelength. A curved gradient using a Thermo Hypersil ODS column
with 0.05% orthophosphoric acid and methanol as mobile phase A and B, respectively, and UV
detection at 277.5 nm was employed. It was observed that a curved gradient along with an optimal
temperature, dramatically improves the signal to noise ratio and separation profile. The limit
of detection (LOD) and limit of quantification (LOQ) were found to be in the range of
1.04–22.81 lgmL�1 and 3.47–76.05 lgmL�1 respectively. The overall precision values obtained
from the analysis of Kangra and Darjeeling orthodox tea samples were within the range of 0–0.34
(standard error).
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1062/1/98_Development_2011.pdf
Sharma, Anupma and Bhondekar, Amol P and Bari, S S and Gulati, Ashu and Kapur, Pawan and Singla, M L (2011) DEVELOPMENT AND OPTIMIZATION OF AN HPLC METHOD FOR THE ROUTINE ANALYSIS OF CATECHINS, CAFFEINE, AND GALLIC ACID IN TEA (CAMELLIA SINENSIS). Journal of Liquid Chromatography & Related Technologies,, 34. pp. 2243-2255.
 
Relation http://ihbt.csircentral.net/1062/