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Selection and differentiation of Bacillus spp. Antagonistic to Fusarium oxysporum f.sp. lycopersici and Alternaria solani infecting Tomato

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Title Selection and differentiation of Bacillus spp. Antagonistic
to Fusarium oxysporum f.sp. lycopersici and Alternaria solani
infecting Tomato
 
Creator Shanmugam, V
Atri, Kamini
Gupta, Samriti
Kanoujia, N
Naruka Singh, Digvijay
 
Subject Plant sciences
Synthetic Chemistry
 
Description Antagonistic Bacillus spp. displaying in vitro
production of siderophore, chitinase, and β-1,3-glucanase
were identified from dual culture assays. In independent
greenhouse studies, seed bacterization and soil application
of Bacillus atrophaeus S2BC-2 challenge inoculated with
Fusarium oxysporum f.sp. lycopersici (FOL) and Alternaria
solani (AS) recorded low percent disease index of 25.3 and
28.7, respectively, over nonbacterised pathogen control
(44.3 and 56.4). The low disease incidence corroborated
with tomato growth promotion with high vigor index
(8,041.2) and fresh plant weight (82.5 g) on challenge
inoculation with FOL. Analysis of root and leaf samples in
rhizobacterial treatment challenged with FOL and AS
revealed maximum induction of chitinase (1.9 and 1.7 U/
mg of protein, respectively) and β-1,3-glucanase (23.5 and
19.2 U/mg of protein, respectively). In native gel activity
assays, the rhizobacterial treatment on challenge inoculation
strongly expressed three high intensity PO isoforms
along with one low intensity isoform. In studies on genetic
diversity of the Bacillus strains by repetitive extragenomic
palindromic-polymerase chain reaction (REP-PCR) and
amplified rDNA restriction analysis (ARDRA) patterns,
ARDRA was more highly discriminant than REP-PCR and
allowed grouping of the strains and differentiation of the
antagonistic strains from other isolates
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1064/1/102_Selection_and_differentiation_2011.pdf
Shanmugam, V and Atri, Kamini and Gupta, Samriti and Kanoujia, N and Naruka Singh, Digvijay (2011) Selection and differentiation of Bacillus spp. Antagonistic to Fusarium oxysporum f.sp. lycopersici and Alternaria solani infecting Tomato. Folia Microbiol, 56. pp. 170-177.
 
Relation http://ihbt.csircentral.net/1064/