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In vitro cormlet production and growth evaluation under greenhouse conditions in saffron (Crocus sativus L.) – A commercially important crop

IR@CSIR-IHBT

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Title In vitro cormlet production and growth
evaluation under greenhouse conditions in
saffron (Crocus sativus L.) – A commercially
important crop
 
Creator Devi, K
Sharma, Madhu
Singh, M K
Ahuja, Paramvir Singh
 
Subject Plant sciences
Nutraceuticals
 
Description The present study describes the effects of season on initial bud sprouting,
direct shoot regeneration from the base of the sprouted bud, and cormlet
production from multiple shoots, and provides growth evaluation of in vitro
produced cormlets under greenhouse conditions. Initial sprouting of buds
from corm segments was previously described in medium supplemented with 2,4-
dichlorophenoxyacetic acid (2,4-D, 9.05 mM) and 6-benzylaminopurine (BAP,
26.64 mM). Maximum bud sprouting (90%) was observed during November and
December. Direct multiple shoot primordia were initiated from the base of these
sprouted buds on BAP (26.64 mM). Multiplication of shoots was achieved in
BAP (26.64 mM) and a-naphthalene acetic acid (1.0 and 5.0 mM). Growth
retardants (chlorocholine chloride and paclobutrazol) were used for cormlet
production from multiple shoots, and paclobutrazol (1.7 mM) evinced maximum
cormlet production (86.07%). Growth of these in vitro-produced cormlets
was evaluated under greenhouse conditions, and 91.66% sprouting was
observed. An increase in cormlet weight (66.88%) was also observed under in vivo
conditions.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1069/1/2011.pdf
Devi, K and Sharma, Madhu and Singh, M K and Ahuja, Paramvir Singh (2011) In vitro cormlet production and growth evaluation under greenhouse conditions in saffron (Crocus sativus L.) – A commercially important crop. Eng. Life Sci., 11 (2). pp. 189-194.
 
Relation http://ihbt.csircentral.net/1069/