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Agrobacterium-Mediated Silencing of Caffeine Synthesis through Root Transformation in Camellia sinensis L.

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Title Agrobacterium-Mediated Silencing of Caffeine Synthesis through
Root Transformation in Camellia sinensis L.
 
Creator Mohanpuria, P
kumar, Vinay
Ahuja, Paramvir Singh
Yadav , S K
 
Subject Plant sciences
Plant Biotechnology
 
Description Tea [Camellia sinensis (L.) O. Kuntze] is a
perennial and most popular non-alcoholic caffeinecontaining
beverage crop. Tea has several constraints for
its genetic improvement such as its high polyphenolic
content and woody perennial nature. The development of
transgenic tea is very difficult, laborious, and time taking
process. In tea, regeneration requires minimum
8–12 months. In view of this, attempt has been made in this
article to develop a rapid, efficient, and quite economical
Agrobacterium-mediated root transformation system for
tea. The feasibility of the developed protocol has been
documented through silencing caffeine biosynthesis. For
this, one-month-old tea seedlings were exposed to fresh
wounding at the elongation zone of roots and were inoculated
with Agrobacterium tumefaciens cultures carrying a
RNAi construct (pFGC1008-CS). The pFGC1008-CS
contained 376 bp of caffeine synthase (CS) cDNA fragment
in sense and antisense direction with an intron in
between. This has made the RNAi construct to produce a
hairpin RNA (ihpRNA). The suppressed expression of CS
gene and a marked reduction in caffeine and theobromine
contents in young shoots of tea seedlings were obtained
after root transformation through Agrobacterium infiltration.
Such transformation system could be useful for
functional analysis of genes in tea like woody and perennial
plants.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1075/1/130_Agrobacterium_26-4-2012.pdf
Mohanpuria, P and kumar, Vinay and Ahuja, Paramvir Singh and Yadav , S K (2011) Agrobacterium-Mediated Silencing of Caffeine Synthesis through Root Transformation in Camellia sinensis L. Mol Biotechnol, 48. pp. 235-243.
 
Relation http://ihbt.csircentral.net/1075/