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Identification of miR414 and Expression Analysis of Conserved miRNAs from Stevia rebaudiana

IR@CSIR-IHBT

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Title Identification of miR414 and Expression Analysis of Conserved
miRNAs from Stevia rebaudiana
 
Creator Guleria, Praveen
Yadav , S K
 
Subject Plant sciences
Plant Biotechnology
 
Description MicroRNAs (miRNAs) usually contain 19-24 nucleotides and have been identified as important eukaryotic gene
regulators. Applications of various computational approaches have simplified the task by predicting miRNAs from
available sequence data sources. In this study, we identified a conserved miR414 from a computational analysis of
EST sequence data available from Stevia rebaudiana. In addition, we also identified six conserved miRNAs
namely miR169, miR319, miR414, miR164, miR167 and miR398 using stem-loop RT-PCR analysis. Hence,
miR414 was commonly identified using both methods. The expression analysis of these miRNAs documented
their roles in growth and development of Stevia. Furthermore, the detected miRNAs were found to target genes
involved in plant growth, development, metabolism and signal transduction. This is the first study reporting these
conserved miRNAs and their expression in Stevia.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1080/1/124_Identification_26-4-2012.pdf
Guleria, Praveen and Yadav , S K (2011) Identification of miR414 and Expression Analysis of Conserved miRNAs from Stevia rebaudiana. Genomics Proteomics Bioinformatics, 9 (6). pp. 211-217.
 
Relation http://ihbt.csircentral.net/1080/