Record Details

A genetic discontinuity in root-nodulating bacteria of cultivated pea in the Indian trans-Himalayas

IR@CSIR-IHBT

View Archive Info
 
 
Field Value
 
Title A genetic discontinuity in root-nodulating bacteria
of cultivated pea in the Indian trans-Himalayas
 
Creator Rahi, P
Kapoor, Riti
Young, J.P.W.
Gulati, Arvind
 
Subject Plant sciences
Microbiology
 
Description Evolutionary relationships of 120 root-nodulating bacteria isolated from the nodules of
Pisum sativum cultivated at 22 different locations of the trans-Himalayan valleys of
Lahaul and Spiti in the state of Himachal Pradesh of India were studied using 16S rRNA
gene PCR-RFLP, ERIC-PCR, sequencing of 16S rRNA, atpD, recA, nodC and nifH genes,
carbon-source utilization pattern (BIOLOG�), and whole-cell fatty acid profiling. The
results demonstrated that all isolates belonged to Rhizobium leguminosarum symbiovar
viciae (Rlv). Isolates from the two valleys were clearly separated on the basis of ERIC
fingerprints, carbon-source utilization pattern, and whole-cell fatty acid methyl esters.
Phylogenetic analysis of atpD, recA, nodC and nifH genes revealed a common Rlv
sublineage in Spiti valley. Lahaul valley isolates were represented by three sequence
types of atpD and recA genes, and four sequence types of nodC and nifH genes.
Genotypes from the two valleys were completely distinct, except for two Lahaul isolates
that shared nodC and nifH sequences with Spiti isolates but were otherwise more similar
to other Lahaul isolates. Isolates from the two highest Spiti valley sites (above 4000 m)
had a distinctive whole-cell fatty acid profile. Spiti valley isolates are closely related to
Rlv sublineages from Xinjiang and Shanxi provinces in China, while Lahaul valley
isolates resemble cosmopolitan strains of the western world. The high mountain pass
between these valleys represents a boundary between two distinct microbial populations
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1086/1/48_A_genetic.pdf
Rahi, P and Kapoor, Riti and Young, J.P.W. and Gulati, Arvind (2012) A genetic discontinuity in root-nodulating bacteria of cultivated pea in the Indian trans-Himalayas. Molecular Ecology, 21. pp. 145-159.
 
Relation http://ihbt.csircentral.net/1086/