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Velvet bean severe mosaic virus: a distinct begomovirus species causing severe mosaic in Mucuna pruriens (L.) DC

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Title Velvet bean severe mosaic virus: a distinct begomovirus species
causing severe mosaic in Mucuna pruriens (L.) DC
 
Creator Zaim , M
Kumar , Y
Hallan , Vipin
Zaidi , A A
 
Subject Plant sciences
Plant viruses
 
Description Velvet bean [Mucuna pruriens (L.) DC] is one
of the most important medicinal plants. It is used to treat
many ailments, but is widely used for the treatment especially
for Parkinson’s disease because of the presence of
3,4-dihydroxyphenylalanine (L-dopa) in it. It was noticed in
last 5 years that the plants in the field showed severe
mosaic, downward curling of the leaves, stunting, etc. This
is consistently observed over the years in India. The disease
was transmitted by whiteflies and by grafting and the causal
agent was found to be a bipartite begomovirus. The whole
genome was amplified by rolling circle amplification
(RCA) using /-29 DNA polymerase and characterized.
DNA-A and DNA-B shared a 124-nucleotide (nt) long
highly conserved (98%) common region (CR). Comparisons
with other begomovirus showed that DNA-A sequence
has highest identity (76%) with an isolate of Mungbean
yellow mosaic India virus (MYMIV; AY937195) reported
from India. This data suggested that the present isolate is a
new species of genus Begomovirus for which the name
‘‘Velvet bean severe mosaic virus’’ (VbSMV) is proposed.
DNA-B has a maximum sequence identity of 49% with an
isolate of Horsegram yellow mosaic virus (HgYMV;
AM932426) reported from India. Infectious clones consisting
of a 1.7 mer partial tandem repeat of DNA-A and a
dimer of DNB-B were constructed and agro-inoculated to
Macuna pruriens (L.) DC plants, which showed field
observed symptoms 24 days post-infiltration (dpi). In phylogenetic
analysis, DNA-A and DNA-B of the present isolate
grouped with DNA-A of different begomoviruses
reported from fabaceous crops. The study presents first ever
molecular evidence of any disease in velvet bean and whole
genome analysis of the causative virus which is a distinct
bipartite species of Begomovirus.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1097/1/25_Velvet.pdf
Zaim , M and Kumar , Y and Hallan , Vipin and Zaidi , A A (2011) Velvet bean severe mosaic virus: a distinct begomovirus species causing severe mosaic in Mucuna pruriens (L.) DC. Virus Genes, 43 (1). pp. 138-146.
 
Relation http://ihbt.csircentral.net/1097/