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IDENTIFICATION AND CROSS-SPECIES TRANSFERABILITY OF 112 NOVEL UNIGENE-DERIVED MICROSATELLITE MARKERS IN TEA ( CAMELLIA SINENSIS )

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Title IDENTIFICATION AND CROSS-SPECIES TRANSFERABILITY OF 112
NOVEL UNIGENE-DERIVED MICROSATELLITE MARKERS IN TEA
( CAMELLIA SINENSIS )
 
Creator Sharma , H
Kumar, R
Sharma , V
Kumar , V
Bhardwaj, P
Ahuja , Paramvir Singh
Sharma, R K
 
Subject Plant sciences
 
Description Premise of the study: Tea Unigene-derived MicroSatellite (TUGMS) markers were identifi ed from the publicly available EST
data in Camellia sinensis for characterization and future genome mapping studies in tea.
• Methods and Results: One hundred twelve novel TUGMS markers were identifi ed from 4356 unigenes derived by clustering
of 12 788 random ESTs in C. sinensis . Amplifi cation-based validation of the TUGMS loci proved them to be highly polymorphic
[an average (av.) of 5.24 alleles], heterozygous ( H E , av. 0.746; H O , av. 0.566) and informative (PIC, av. 0.392). TUGMS
loci were 100% transferable in cultivated C. assamica and C. assamica subsp. lasiocalyx and highly cross-transferrable to the
related species C. japonica , C. rosifl ora, and C. sasanqua .
• Conclusions: These 112 novel highly polymorphic TUGMS markers with proven cross-species transferability will facilitate the
future genetic diversity and genome mapping studies in tea.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1106/1/41_Identification.pdf
Sharma , H and Kumar, R and Sharma , V and Kumar , V and Bhardwaj, P and Ahuja , Paramvir Singh and Sharma, R K (2011) IDENTIFICATION AND CROSS-SPECIES TRANSFERABILITY OF 112 NOVEL UNIGENE-DERIVED MICROSATELLITE MARKERS IN TEA ( CAMELLIA SINENSIS ). American Journal of Botany, 98 (6). E-133-E-138.
 
Relation http://ihbt.csircentral.net/1106/