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2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Stevia rebaudiana Bertoni is a functional gene

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Title 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Stevia
rebaudiana Bertoni is a functional gene
 
Creator Kumar , Hitesh
Singh , Kashmir
Kumar , Sanjay
 
Subject Plant sciences
Plant Biotechnology
 
Description Stevia [Stevia rebaudiana (Bertoni)] is a
perennial herb which accumulates sweet diterpenoid steviol
glycosides (SGs) in its leaf tissue. SGs are synthesized
by 2C-methyl-D-erythritol 4-phosphate (MEP) pathway. Of
the various enzymes of the MEP pathway, 2C-methyl-Derythritol
2,4-cyclodiphosphate synthase (MDS) (encoded
by MDS) catalyzes the cyclization of 4-(cytidine 50
diphospho)-2C-methyl-D-erythritol 2-phosphate into 2Cmethyl-
D-erythritol 2,4-cyclodiphosphate. Complementation
of the MDS knockout mutant strain of Escherichia
coli, EB370 with putative MDS of stevia (SrMDS) rescued
the lethal mutant, suggesting SrMDS to be a functional
gene. Experiments conducted in plant growth chamber and
in the field suggested SrMDS to be a light regulated gene.
Indole 3-acetic acid (IAA; 50, 100 lM) down-regulated the
expression of SrMDS at 4 h of the treatment, whereas,
abscisic acid did not modulate its expression. A high
expression of SrMDS was observed during the light hours
of the day as compared to the dark hours. The present work
established functionality of SrMDS and showed the role of
light and IAA in regulating expression of SrMDS.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1199/1/1_2C-methyl-D-erythritol.pdf
Kumar , Hitesh and Singh , Kashmir and Kumar , Sanjay (2012) 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Stevia rebaudiana Bertoni is a functional gene. Mol Biol Rep, 39. pp. 10971-10978.
 
Relation http://ihbt.csircentral.net/1199/