Record Details

Development of eco-friendly bioplastic like PHB by distillery effluent microorganisms

IR@CSIR-IHBT

View Archive Info
 
 
Field Value
 
Title Development of eco-friendly bioplastic like PHB by distillery
effluent microorganisms
 
Creator Gangurde, N S
Sayyed , R S
Kiran , S
Gulati, Arvind
 
Subject Microbiology
 
Description During screening for poly-β-hydroxybutyrate
(PHB) producing bacteria from distillery effluent sample,
six out of 30 isolates comprising of three strains of
Alcaligenes sp., two strains of Bacillus sp., and one
strain of Pseudomonas sp. were found to accumulate
varying levels of intracellular PHB. Amongst the various
isolates, Alcaligenes sp. RZS4 was found as the
potent PHB-producing organism, accumulating higher
amounts of PHB. PHB productivity was further enhanced
in the presence of oxygen, nitrogen-limiting
conditions, and cloning of PHB synthesizing genes of
Alcaligenes sp. RZS 4 into Escherichia coli. A twofold
increase in PHB yield was obtained from recombinant
E. coli vis-à-vis Alcaligenes sp.; the recombinant E. coli
accumulated more PHB in NDMM, produced good
amount of PHB in a single-stage cultivation process
under both nutrient-rich and nutrient-deficient conditions.
Extraction of PHB with acetone–alcohol (1:1)
was found as suitable method for optimum extraction
of PHB as this mixture selectively extracted PHB without
affecting the non-PHB cell mass. PHB extract from
recombinant E. coli showed the presence of C–H, 0O
stretching, 0C–H deformation, 0C–H, 0CH, and 0C–O
functional groups characteristic of PHB.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1209/1/12_Development.pdf
Gangurde, N S and Sayyed , R S and Kiran , S and Gulati, Arvind (2012) Development of eco-friendly bioplastic like PHB by distillery effluent microorganisms. Environmental Science and Pollution Research.
 
Relation http://ihbt.csircentral.net/1209/