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Differentially expressed transcripts from leaf and root tissue of Chlorophytum borivilianum: A plant with high medicinal value

IR@CSIR-IHBT

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Title Differentially expressed transcripts from leaf and root tissue of Chlorophytum
borivilianum: A plant with high medicinal value
 
Creator Kumar , S
Kalra, S
Kumar, Sanjay
Kaur, J
Singh, K
 
Subject Plant sciences
Plant Biotechnology
 
Description Chlorophytum borivilianum is one of the important medicinal plants used for treating different health problems
such as diabetes, arthritis, physicalweakness, etc. Saponins present in C. borivilianumare the primary source of its
significant medicinal properties and are synthesized by mevalonate and non-mevalonate pathways in plants.
However, the biosynthesis of these compounds at molecular level is not studied in C. borivilianum. Cloning and
sequencing of genes involved inmetabolic processes are prerequisite to study the gene expression, their regulation
and genetic engineering experiments. Expressed sequence tags (ESTs) provide a quick insight into various
genes and their tissue specific expression. Suppression subtractive hybridization (SSH) libraries were
constructed using mRNA from leaf and root tissues of C. borivilianum. High quality non‐redundant 506 and 303
ESTswere generated fromleaf and root specific libraries respectively. These sequences were analyzed using bioinformatics
tools and grouped into different categories based on their similarity and cellular functions such as
photosynthesis, metabolism, transcription factors, cell signaling, defense, stress response etc. ESTs also showed
similarity with genes involved in saponins biosynthesis such as squalene synthase, squalene epoxidase, cytochrome
p450, glycosyltransferase, etc. Semi-quantitative analysis of some of the ESTs involved in saponins biosynthesis
confirmed their differential regulation in leaves and roots. These ESTs will provide an efficient resource to accelerate
gene discovery in C. borivilianum and will help in determining promising targets for genetic engineering of
saponins pathway.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1211/1/14_Differentially.pdf
Kumar , S and Kalra, S and Kumar, Sanjay and Kaur, J and Singh, K (2012) Differentially expressed transcripts from leaf and root tissue of Chlorophytum borivilianum: A plant with high medicinal value. Gene.
 
Relation http://ihbt.csircentral.net/1211/