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Engineering a thermo-stable superoxide dismutase functional at sub-zero to .50uC, which also tolerates autoclaving

IR@CSIR-IHBT

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Title Engineering a thermo-stable superoxide
dismutase functional at sub-zero to
.50uC, which also tolerates autoclaving
 
Creator Kumar, A
Dutt, Som
Bagler, Ganesh
Ahuja, Paramvir Singh
Kumar, Sanjay
 
Subject Plant sciences
Natural Product Chemistry
 
Description Superoxide dismutase (SOD) is a critical enzyme associated with controlling oxygen toxicity arising out of
oxidative stress in any living system. A hyper-thermostable SOD isolated from a polyextremophile higher
plant Potentilla atrosanguinea Lodd. var. argyrophylla (Wall. ex Lehm.) was engineered by mutation of a
single amino acid that enhanced the thermostability of the enzyme to twofold. The engineered enzyme was
functional from sub-zero temperature to .506C, tolerated autoclaving (heating at 1216C, at a pressure of
1.1 kg per square cm for 20 min) and was resistant to proteolysis. The present work is the first example to
enhance the thermostability of a hyper-thermostable protein and has potential to application to other
proteins for enhancing thermostability.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1216/1/20_Engineering.pdf
Kumar, A and Dutt, Som and Bagler, Ganesh and Ahuja, Paramvir Singh and Kumar, Sanjay (2012) Engineering a thermo-stable superoxide dismutase functional at sub-zero to .50uC, which also tolerates autoclaving. Scientific Reports (Nature Publishing Group), 2. p. 387.
 
Relation http://ihbt.csircentral.net/1216/