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Simultaneous quantification of Amaryllidaceae alkaloids from Zephyranthes grandiflora by UPLC–DAD/ESI-MS/MS

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Title Simultaneous quantification of Amaryllidaceae alkaloids from Zephyranthes
grandiflora by UPLC–DAD/ESI-MS/MS
 
Creator Katoch, Deepali
Kumar , Shiv
Kumar, Neeraj
Singh , Bikram
 
Subject Natural Product Chemistry
 
Description A rapid, simple and sensitive ultra performance liquid chromatography–diode array detection method
(UPLC–DAD) was developed and validated for quantification of four biologically important Amaryllidaceae
alkaloids viz. lycoramine, hamayne, haemanthamine and tortuosine in Zephyranthes grandiflora.
The method employed BEH C18 column (2.1 mm
×100 mm, 1.7 μ m particle size) with linear gradient elution
of acetonitrile and water (0.05% formic acid) in a flow rate of 0.3 mL/min and at λ max 280 nm. Standard
calibration curve for the analytes were linear (r2 ≥
0.9999), precise (intra-day RSDs < 1.33% and inter-day
RSDs < 2.67%) and accurate (97.8–105.3%). The developed method was applied to the quality assessment
of samples collected during different seasons and showed significant variation with an optimum amounts
of these alkaloids in rainy season. The method was also applied for identification of Amaryllidaceae alkaloids
in the plant and overall, seventeen Amaryllidaceae alkaloids of different structural types lycorine,
haemanthamine, galanthamine, narciclasine were characterised. This study provides a qualitative and
quantitative method for analysis of Amaryllidaceae alkaloids.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1246/1/52_Simultaneous_quantification_of_Amaryllidaceae_alkaloids_from_Zephyranthes.pdf
Katoch, Deepali and Kumar , Shiv and Kumar, Neeraj and Singh , Bikram (2012) Simultaneous quantification of Amaryllidaceae alkaloids from Zephyranthes grandiflora by UPLC–DAD/ESI-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 71. pp. 187-192.
 
Relation http://ihbt.csircentral.net/1246/