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A RAPID HPLC-DAD METHOD FOR ANALYSIS OF THEAFLAVINS USING C12 AS STATIONARY PHASE

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Title A RAPID HPLC-DAD METHOD FOR ANALYSIS OF THEAFLAVINS
USING C12 AS STATIONARY PHASE
 
Creator Rana, Ajay
Singh, H P
 
Subject Plant sciences
 
Description Theaflavins are the major pharmacologically active antioxidant compounds present in the
black tea. In this study we have developed a rapid, simple, and sensitive HPLC-DAD method for
estimation and analysis of theaflavins using a RP-C12(4.6 х 250 mm, 4 micron) column. The
present study aims at rapid estimation and analysis of theaflavins in black tea and black tea based
products (Ready to Drink Teas and Instant Teas) for their qualitative analysis. The use of RP-C12 as
stationary phase with a simple gradient method enables the excellent separation of all the four major
tea theaflavins: theaflavin (TF-1), theaflavin-3-gallate (TF-2), theaflavin-3, 30-digallate (TF-3),
and theaflavin-30-gallate (TF-4) within 7 min. A linear calibration curve (r2¼0.999) was plotted
against standard theaflavin. The purification of theaflavins from black tea (CTC) was done by
reverse phase solid phase extraction (RP-SPE) technique and quantification of individual theaflavins
were carried out as theaflavin equivalent per mg of total purified theaflavins (TFS) from black
tea. The results of present study clearly shows that use of C12 as a new stationary phase owes high
sensitivity and faster analysis of theaflavins that otherwise are not easy to separate within 10 min.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1285/1/64_A_Rapid_HPLC-DAD_H.P_Singh.pdf
Rana, Ajay and Singh, H P (2012) A RAPID HPLC-DAD METHOD FOR ANALYSIS OF THEAFLAVINS USING C12 AS STATIONARY PHASE. Journal of Liquid Chromatography & Related Technologies, 35 (16). pp. 2272-2279.
 
Relation http://ihbt.csircentral.net/1285/