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Somatic Embryogenesis and Agrobacterium Mediated Genetic Transformation in Bamboos

IR@CSIR-IHBT

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Title Somatic Embryogenesis and Agrobacterium Mediated
Genetic Transformation in Bamboos
 
Creator Sood, A
Bhattacharya, Amita
Sharma, Madhu
Sharma, R K
Nadha, Harleen Kaur
Sood, Priyanka
Mehta, R.
Kaur, D
Brar, Jasmine
Ahuja, Paramvir Singh
 
Subject Plant sciences
Plant Biotechnology
 
Description Somatic embryogenesis is a highly preferred method for in vitro multiplication of
plants. It also forms an ideal experimental model for investigating the process of
differentiation and for understanding the mechanism of totipotency in plants. The
phenomenon of recurrent somatic embryogenesis makes these attractive explants for
genetic transformation and production of transgenic plants. Somatic embryogenesis
in monocots has also been reported to reset the internal calendar at zero; thereby,
enabling the germinated plantlets to live their full life span. Moreover, there is an
increasingly high depletion of natural stands of bamboos mainly due to its
gregarious flowering habit and anthopogenic pressures. However, the plant has
emerged as a crop of the future and has a huge potential in paper, wood and textile
industry. In fact, bamboos are among the most popular groups of plants owing to
their immense utility, phenotypic variability and adaptability. Therefore, there is an
urgent need for devising methods for large scale propagation of bamboos
particularly, because the conventional methods of propagation are inefficient and
labour intensive. In this regard, IHBT has demonstrated somatic embryogenesis to be
a promising strategy that has proven to be superior to multiple shoot formation from
nodal explants. The process of somatic embryogenesis in different bamboos, its
application as a popular technology for eco-reforestation and industry; for
production of plants with improved resistance to stresses through transgenic
technology and also for mid-term conservation under liquid paraffin overlay have
been discussed in this chapter. Plants produced from somatic embryogenesis have
also been used as a model to induce in vitro flowering consistently and for
understanding this complex and difficult process in bamboos.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1288/1/66_Somatic_Embryoge_esis.pdf
Sood, A and Bhattacharya, Amita and Sharma, Madhu and Sharma, R K and Nadha, Harleen Kaur and Sood, Priyanka and Mehta, R. and Kaur, D and Brar, Jasmine and Ahuja, Paramvir Singh (2012) Somatic Embryogenesis and Agrobacterium Mediated Genetic Transformation in Bamboos. Journal of (Eds) Junaid Aslam, P.S. Srivastava, M.P. Sharma, Narosa Book Distributors Pvt Ltd. New Delhi,. pp. 168-178.
 
Relation http://ihbt.csircentral.net/1288/