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Modulation of genotoxicity of oxidative mutagens by glycyrrhizic acid from Glycyrrhiza glabra L.

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Title Modulation of genotoxicity of oxidative mutagens by
glycyrrhizic acid from Glycyrrhiza glabra L.
 
Creator Kaur, Prabhjit
Sharma, Neha
Singh , Bikram
Kumar , Subodh
Kaur, Satwinderjeet
 
Subject Plant sciences
Natural Product Chemistry
 
Description Background: The chemopreventive effects of certain phytoconstituents can be exploited for their
use as functional foods, dietary supplements and even as drugs. The natural compounds, acting
as anti-genotoxic and free radical scavenging compounds, may serve as potent chemopreventive
agents. These can inhibit DNA modulatory activities of mutagens and help preventing pathological
processes. Objectives: Present study on Glycyrrhiza glabra L., a promising medicinal plant, widely
used in traditional medicine, focused on the bioassay-guided fractionation of its extracts for the
isolation of certain phytochemicals with anti-genotoxic potential against oxidative mutagens.
Materials and Methods: The methanol extract of Glycyrrhiza glabra rhizomes was subjected to
column chromatography, and isolated fraction was evaluated for its anti-genotoxic and antioxidant
potential using SOS chromotest, Comet assay, and DPPH radical scavenging assay. Results: GLG
fraction, which was characterized as Glycyrrhizic acid, inhibited the genotoxicity of oxidative
mutagens viz., H 2O2 and 4NQOquite efficiently. In SOS chromotest, using E.coli PQ37 tester
strain, it inhibited induction factor induced by H2O2 and 4NQO by 75.54% and 71.69% at the
concentration of 121.46 μM,respectively. In Comet assay, it reduced the tail moment induced
by H2O2 and 4NQO by 70.21% and 69.04%, respectively, at the same concentration in human
blood lymphocytes. The isolated fraction also exhibited DPPH free radical scavenging activity and
was able to scavenge 85.95% radicals at a concentration of 120 μM. Conclusion: Glycyrrhizic
acid is a potential modulator of genotoxins as well as efficient scavenger of free radicals.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1310/1/11_Address_Modulation.pdf
Kaur, Prabhjit and Sharma, Neha and Singh , Bikram and Kumar , Subodh and Kaur, Satwinderjeet (2012) Modulation of genotoxicity of oxidative mutagens by glycyrrhizic acid from Glycyrrhiza glabra L. Journal of Pharmacognosy Research, 4 (4). pp. 189-195.
 
Relation http://ihbt.csircentral.net/1310/