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Protein changes during ethanol induced seed germination in Aconitum heterophyllum

IR@CSIR-IHBT

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Title Protein changes during ethanol induced seed germination in Aconitum
heterophyllum
 
Creator Rana, Bindu
Sreenivasulu, Y
 
Subject Plant Biotechnology
 
Description Aconitum heterophyllum is a high altitude medicinal plant that has become endangered due to overexploitation
for their aconitins. The most effective, conventional propagation method for any plant species
is by seed. However, in Aconitum seed germination is erratic, and seedling survival is low. In the present
study results have been discussed on the possible implication of ethanol treatment on removal of barriers
on radical emergence in terms of protein changes. Eighty seven percent of seed germination was achieved
in Aconitum with ethanol treatment. Comparative 2-DE analysis of ethanol treated and untreated seed
protein profiles in Phase II of germination revealed 40 differentially expressed proteins. Twenty-seven out
of 40 proteins were induced, 5 were increased and 8 were repressed. Mass spectrometry and subsequent
identification confirmed that these proteins were involved in metabolism, DNA regulation, stress tolerance
and plasmamembrane/cell wall biosynthesis/extension processes. These protein changes might be
responsible for physiological and physical changes, respectively, resulted in increase in germination percentage.
Further, characterization of these proteins will be of great help in understanding the molecular
mechanism lying behind enhanced germination in response to ethanol treatment
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1327/1/124_Protein_changes.pdf
Rana, Bindu and Sreenivasulu, Y (2013) Protein changes during ethanol induced seed germination in Aconitum heterophyllum. Journal of Plant Science, 198. pp. 27-38.
 
Relation http://ihbt.csircentral.net/1327/