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Change in protein content during seed germination of a high altitude plant Podophyllum hexandrum Royle

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Title Change in protein content during seed germination of a high
altitude plant Podophyllum hexandrum Royle
 
Creator Dogra, Vivek
Ahuja, Paramvir Singh
Sreenivasulu, Y
 
Subject Plant Biotechnology
 
Description Podophyllum hexandrum Royle (=Sinopodophyllum hexandrum) is a high-altitude medicinal
plant exploited for its etoposides which are potential anticancer compounds. An effective,
conventional propagation method is by seed. However, seed germination is erratic, and
seedling survival is low. A marginal increase in Podophyllum seed germination was
attained with organic solvents. In the present study an attempt was made to decipher the
physiological and biochemical barriers in terms of change in proteins during seed
germination of Podophyllum. Comparative 2-DE analysis between un-germinated
(dormant) and germinating seeds revealed nearly 113 differentially expressed proteins,
whereas Peptide Mass Fingerprint (PMF) analysis of 97 protein spots revealed appearance of
27 proteins, up-accumulation of 11 proteins, down-accumulation of 19 proteins and
disappearance of 40 proteins with germination. Identified 59 proteins in the homology
search were involved in metabolism (carbohydrate and amino acid metabolism; 20
proteins), ABA/GA signaling (17 proteins) and stress (15 proteins) related proteins. Seven
proteins were with unknown function. Two-DE, and MS/MS analysis in conjunction with
semi-quantitative RT-PCR data of cell wall hydrolyzing genes, revealed that in Podophyllum
the radicle protrusion occurs might be because of the up-accumulation of cell wall
hydrolases i.e. β-1, 3-glucanase and XET which weakens the thick walled micropylar
endosperm.
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1328/1/125_Change_in_Protein.pdf
Dogra, Vivek and Ahuja, Paramvir Singh and Sreenivasulu, Y (2013) Change in protein content during seed germination of a high altitude plant Podophyllum hexandrum Royle. Journal of Proteomics, 78. pp. 26-38.
 
Relation http://ihbt.csircentral.net/1328/