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In vitro cytotoxicity, antimicrobial, and metal-chelating activity of triterpene saponins from tea seed grown in Kangra valley, India

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Title In vitro cytotoxicity, antimicrobial, and metal-chelating activity
of triterpene saponins from tea seed grown in Kangra valley, India
 
Creator Joshi, Robin
Sood, Swati
Dogra, Poonam
Mahendru, Madhvi
Kumar, Dharmesh
Bhangalia, Shalika
Pal, Harish Chandra
Kumar, Neeraj
Bhushan, Shashi
Gulati, Arvind
Saxena, Ajit Kumar Saxena
Gulati, Ashu
 
Subject Plant sciences
 
Description This study was undertaken to isolate and
characterize saponins from seeds of Camellia sinensis.
Four triterpene saponins S1, S2, S3, and S4 were isolated by
chromatography on silica (60–120 mesh), followed by
purification on Sep-Pak C-18 columns. The chemical
structures (S1–S4) were elucidated on the basis of 1-D and
2-D NMR. All the saponins show broad-spectrum antifungal
activity against Candida albicans, Issatchenkia
orientalis, Aspergillus flavus, A. niger, A. ochraceous, A.
parasiticus, A. sydowii, and Trichophyton rubrum. The
most susceptible test fungus was T. rubrum inhibited at a
minimum inhibitory concentration of 31.25 lg/ml by all
the four saponins. Cytotoxicity of these saponins was
evaluated by methyl thiazole tetrazolium and sulfo-rhodamine
B assays. The saponins when tested against five
human cancer cells lines, viz., OVCAR-5 (ovarian carcinoma
cells), MCF-7 (human breast adenocarcinoma cells),
PC-3 (human prostate cancer cells), Colo-205 (colorectal
adenocarcinoma cells), and HL-60 (human promyelocytic
leukemia cells) showed high cytotoxicity activity (99 %)
by S1 and S2 on PC-3 cells at concentration of 100 lg/ml.
Similarly, when these saponins were tested against human
PBMCs by lymphocytes proliferation assay, none showed
significant activity. S3 (IC50 = 1.72 mg/ml) showed high
metal-chelating activity at a concentration of 20 mg/ml.
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1331/1/113_In_vitro_cytotoxicity.pdf
Joshi, Robin and Sood, Swati and Dogra, Poonam and Mahendru, Madhvi and Kumar, Dharmesh and Bhangalia, Shalika and Pal, Harish Chandra and Kumar, Neeraj and Bhushan, Shashi and Gulati, Arvind and Saxena, Ajit Kumar Saxena and Gulati, Ashu (2013) In vitro cytotoxicity, antimicrobial, and metal-chelating activity of triterpene saponins from tea seed grown in Kangra valley, India. Journal of Medicinal Chemistry Research.
 
Relation http://ihbt.csircentral.net/1331/