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Use of chitinolytic Bacillus atrophaeus strain S2BC-2 antagonistic to Fusarium spp. for control of rhizome rot of ginger

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Title Use of chitinolytic Bacillus atrophaeus strain S2BC-2
antagonistic to Fusarium spp. for control of rhizome
rot of ginger
 
Creator Thakur , Himankshi
Shanmugam, V
Gupta, Samriti
 
Subject Plant sciences
 
Description An antagonistic bacillus S2BC-2 isolated from
apple rhizosphere soil was identified to be highly chitinolytic
on chitinase detection agar. Standard bacteriological
tests and sequencing of 16S rRNA, and gyrA and rpoB
genes, indicated a taxonomic affiliation of the strain to
Bacillus atrophaeus. The strain was studied for its ability
to grow and produce chitinase on different substrates.
Bacterial cells grown on chitin-containing media showed
enhanced growth and chitinase production with increased
anti-fungal activity against vascular wilt pathogens.
Extracellular proteins of cell-free extracts of media amended
with chitin and fungal cell wall contained 4–10 novel polypeptides.
In polyhouse (bamboo structures that provide protective
shade made of polyvinyl sheet) studies, a chitinsupplemented
talc-based formulation of the S2BC-2 challenge
inoculated with Fusarium oxysporum f. sp. zingiberi
recorded low percent disease indices of 84.9 % and 79.2 %
for yellows and rhizome rot, respectively, over the nonbacterised
pathogen control. The low disease incidences
correlated with 113.3 % maximum rhizome production and
2-fold higher chitinase induction over the pathogen control.
In native gel activity assays, upon challenge-inoculation,
S2BC-2 expressed more chitinase isoforms than the pathogen
control. The results suggest that chitinolytic B. atrophaeus
can be used in the biocontrol of rhizome rot of
ginger.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1334/1/126_Use_of.pdf
Thakur , Himankshi and Shanmugam, V and Gupta, Samriti (2012) Use of chitinolytic Bacillus atrophaeus strain S2BC-2 antagonistic to Fusarium spp. for control of rhizome rot of ginger. Journal of Annals of Microbiology.
 
Relation http://ihbt.csircentral.net/1334/