Antioxidant activity and identification of bioactive compounds from leaves of Anthocephalus cadamba by ultra-performance liquid chromatography/electrospray ionization quadrupole time of flight mass spectrometry
IR@CSIR-IHBT
View Archive InfoField | Value | |
Title |
Antioxidant activity and identification of bioactive compounds from leaves of Anthocephalus cadamba by ultra-performance liquid chromatography/electrospray ionization quadrupole time of flight mass spectrometry |
|
Creator |
Chandel, Madhu
Sharma, Upendra Kumar, Neeraj Singh , Bikram |
|
Subject |
Natural Product Chemistry
|
|
Description |
Objective: To evaluate the antioxidant potential of different extract/fractions of Anthocephalus cadamba (A. cadamba) (Roxb.) Miq. (Rubiaceae) and study the tentative identification of their active constituents. Methods: The extract/fractions were screened for antioxidant activity using various in vitro assays viz. DPPH assay, ABTS assay, superoxide anion radical scavenging assay, wreadsu cdientge rpmoiwneerd a bssya cy oalnordi mpleatsrmici dm DetNhAod n. icAkni nugl tarsas-apye. rTfoortaml apnhceen oLlCic- ecloenctternots porf aeyx-trqaucat/dfrraucptoiolen-s tfirmacet ioonf sf liogf hAt . mcaadssa mspbeac. trRoemseutlrtys :m Tehteh oedth wyla sa cuesteadte tfor aacntiaolny swe atsh efo aucntdiv teo cboen mstiotsute anctsti voef ferxatcrtaicotn/ (iEn AaAllC t hfrea catsisoany)s w aass c2o1m.2p4a red to other extract/fractions. The IC50 value of ethyl acetate fraction µg/mL, 1.12 ug/mL, 9.68 µg/mL and 57.81 µg/mL in DPPH assay, AfrBacTtSio ansss aayl,s ore sdhuocwinegd ptohwe epr oatsesnatyia al ntdo spurpoteercotx itdhee spclaavsemnigdi ng assay respectively. All the extract/ hydroxyl radicals generated by DNA (pBR322) against the attack of Fenton’s reagent. The bioactive compounds were identified by UPLC-ESI-QTOF-MS, by comparing the mass and λmax with literature values. Conclusions: The pthoatet nthtieayl mofa tyh eb ee xutsreafcutl/ ftrhaecrtaiopnesu ttioc sacgaevnetnsg feo rd tirfefearteinngt frraedei craald-icreallast eind dpiaftfheroelongt iscy dstaemmasg ein.dicated |
|
Date |
2012
|
|
Type |
Article
PeerReviewed |
|
Format |
application/pdf
|
|
Identifier |
http://ihbt.csircentral.net/1349/1/136_Antioxidant.pdf
Chandel, Madhu and Sharma, Upendra and Kumar, Neeraj and Singh , Bikram (2012) Antioxidant activity and identification of bioactive compounds from leaves of Anthocephalus cadamba by ultra-performance liquid chromatography/electrospray ionization quadrupole time of flight mass spectrometry. Asian Pacific Journal of Tropical Medicine. pp. 977-985. |
|
Relation |
http://ihbt.csircentral.net/1349/
|
|