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In vitro propagation of Dendrobium aphyllum (Orchidaceae)—seed germination to flowering

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Title In vitro propagation of Dendrobium aphyllum (Orchidaceae)—seed
germination to flowering
 
Creator Hossain, M M
Sharma, Madhu
Pathak, Promila
 
Subject Plant sciences
Plant Biotechnology
 
Description This communication describes asymbiotic seed
germination, protocorm development, micropropagation
and flowering in in vitro and hardened seedlings of Dendrobium
aphyllum (Roxb.) C.E.C. Fischer. Effects of four
culture media viz., Murashige and Skoog (MS); Phytamax
(Sigma Chemical Co. USA; PM); Mitra et al. (M) and
Knudson ‘C’ (KC), 6-benzylaminopurine (BAP) and 2,4-
dichlorophenoxyacetic acid (2,4-D), peptone and activated
charcoal were studied on seed germination and protocorm
development. Maximum germination (97 %) was recorded
in PM basal medium. Peptone (2.0 gl−1) remarkably enhanced
germination percentage (100 %), vigorous growth,
high survival and subsequent development of protocorms,
while in activated charcoal the response was not encouraging.
BAP improved germination percentage, however, 2,4-D
showed noticeably low seed germination. The morphogenetic
response of protocorms and nodal segments of in vitro
raised seedlings varied depending on type of explants and
concentrations and combinations of plant growth regulators
used. Stout root system was induced in 1/2PM + 0.5 mgl−1
IAA. Approximately 10 % of the in vitro raised plants (4–
5 cm) with 3–4 leaves flowered in vitro irrespective of
flowering season. The well-rooted plants showed 80 % survival
under green house conditions and flowering was noticed
after 5–6 months in 10 % of hardened plants.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/1353/1/139_In_vitro.pdf
Hossain, M M and Sharma, Madhu and Pathak, Promila (2012) In vitro propagation of Dendrobium aphyllum (Orchidaceae)—seed germination to flowering. Journal of Plant Biochemistry Biotechnology.
 
Relation http://ihbt.csircentral.net/1353/