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Increased nutritive value of transgenic potato by expressing a nonallergenic seed albumin gene from Amaranthus hypochondriacus

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Title Increased nutritive value of transgenic potato by expressing a nonallergenic seed albumin gene from Amaranthus hypochondriacus
 
Creator Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Amaranthus hypochondriacus
transgenic potato
albumin gene
AmA1
nutritional improvement of potato tubers
 
Description Improvement of nutritive value of crop plants, in particular the
amino acid composition, has been a major long-term goal of plant
breeding programs. Toward this end, we reported earlier the
cloning of the seed albumin gene AmA1 from Amaranthus hypo-
chondriacus. The AmA1 protein is nonallergenic in nature and is
rich in all essential amino acids, and the composition corresponds
well with the World Health Organization standards for optimal
human nutrition. In an attempt to improve the nutritional value of
potato, the AmA1 coding sequence was successfully introduced
and expressed in tuber-specific and constitutive manner. There was
a striking increase in the growth and production of tubers in
transgenic populations and also of the total protein content with
an increase in most essential amino acids. The expressed protein
was localized in the cytoplasm as well as in the vacuole of
transgenic tubers. Thus we have been able to use a seed albumin
gene with a well-balanced amino acid composition as a donor
protein to develop a transgenic crop plant. The results document,
in addition to successful nutritional improvement of potato tubers,
the feasibility of genetically modifying other crop plants with
novel seed protein composition.
 
Date 2013-10-07T08:43:40Z
2013-10-07T08:43:40Z
2000
 
Type Article
 
Identifier Proc. Natl. Acad. Sci. USA, 97: 3724-3729
http://hdl.handle.net/123456789/13
 
Language en
 
Publisher Proceedings of the National Academy of Sciences of the United States of America