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A secretion signal is present in the Collybia velutipes oxalate decatboxylase gene

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Title A secretion signal is present in the Collybia velutipes oxalate decatboxylase gene
 
Creator Azam, Mohammad
Kesarwani, Meenu
Natarajan, Krishnamurthy
Datta, Asis
 
Subject Collybia velutipes
oxalate decarboxylase
Saccharomyces
secretion signal
expression vector
Schizosaccharomyces pombe
 
Description The oxalate decarboxylase (OXDC) gene from Collybia velutipes is overexpressed as an active form in Schizosaccharomyces pombe. The recombinant enzyme shows similar pH optima and stability, while
substrate kinetic analysis shows a ninefold decrease
in K m value with respect to native OXDC. Most of the
expressed protein was present in periplasm and remained firmly bound to cell-wall materials. However,
20% of enzyme expressed was secreted out into the
medium suggesting the presence of a secretion signal
(C. velutipes) in the oxalate decarboxylase gene. This
secretion signal is associated with the N-terminal of
OXDC as is evident by secretion of nonsecretory genes
AmA1 and Beta-galactosidase. An expression vector using this signal is constructed for expression and secretion of heterologous proteins in S. pombe.
This work was supported by a grant from the Department of Biotechnology, Ministry of Science and Technology, Government of India.
 
Date 2013-10-09T05:35:38Z
2013-10-09T05:35:38Z
2001
 
Type Article
 
Identifier Biochem. Biophys. Res. Comm., 289(4): 807-812
http://hdl.handle.net/123456789/15
 
Language en
 
Publisher Elsevier B.V.