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Plant pre-mRNA splicing in fission yeast, Schizosaccharomyces pombe

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Title Plant pre-mRNA splicing in fission yeast, Schizosaccharomyces pombe
 
Creator Sarmah, Bhaskarjyoti
Chakraborty, Niranjan
Chakraborty, Subhra
Datta, Asis
 
Subject Seed-specific gene
AmA1
Schizosaccharomyces pombe
Intron recognition
Gene splicing
 
Description Pre-mRNA splicing or the removal of introns from precursor messenger RNAs depends on the accurate recognition of intron
sequences by the splicing machinery. We have analyzed various aspects of intron sequence and structure in relation to splice site
selection and splicing efficiency of a plant gene AmA1 in Schizosaccharomyces pombe. Earlier, we reported the cloning of AmA1, a
seed albumin gene from Amaranthus hypochondriacus [A. Raina, A. Datta, Proc. Natl. Acad. Sci. USA 89 (1992) 11774]. In the
absence of an in vitro splicing system for plants, the expression of AmA1 genomic clone in S. pombe has been used to analyze splicing
of intron constructs. We aim to focus on S. pombe as a possible alternative and examined its effectiveness as a host for plant gene
splicing. The results show here that pre-mRNA transcripts of AmA1 gene underwent splicing in S. pombe. Ó 2002 Elsevier Science
(USA). All rights reserved.
 
Date 2013-10-21T06:19:13Z
2013-10-21T06:19:13Z
2002
August 2002
 
Type Article
 
Identifier Biochem. Biophys. Res. Comm., 293: 1209-1216
http://hdl.handle.net/123456789/27
 
Language en
 
Publisher Academic Press