Record Details

Light regulated modulation of Z-box containing promoters by photoreceptor and downstream regulatory components, COP1 and HY5 in Arabidopsis thaliana

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Light regulated modulation of Z-box containing promoters by photoreceptor and downstream regulatory components, COP1 and HY5 in Arabidopsis thaliana
 
Creator Yadav, Vandana
Kundu, Snehangshu
Chattopadhyay, Debasis
Negi, Prem
Wei, Ning
Deng, Xing-Wang
Chattopadhyay, Sudip
 
Subject Z-box
ZBF
LRE
HY5
COP1
photoreceptor
 
Description The Z-box is one of the light-responsive elements (LREs) found in the promoters of light inducible genes.
We have studied the light responsive characteristics of Z-box containing synthetic as well as native
promoters. We show that promoters with Z-box as a single LRE or paired with another LRE can respond
to a broad spectrum of light. The response is primarily mediated by phyA, phyB and CRY1
photoreceptors at their respective wavelengths of light. We have demonstrated that CAB1 and Z-GATA
containing promoters are down-regulated in hy5 mutants in the light. On the other hand, a promoter
with Z-box alone is down-regulated in hy5 mutants both in dark and in light conditions, suggesting
involvement of a similar regulatory system in the regulation of the promoter in two distinct
developmental pathways: skotomorphogenesis and photomorphogenesis. Furthermore, similar to the
CAB1 promoter, a Z-GATA containing promoter is derepressed in cop1 mutants in the dark. DNA±protein
interaction studies reveal the presence of a DNA-binding activity that is speci®c to Z-box. These results
provide insights into the regulation of the Z-box LRE mediated by various light signaling components.
This work was supported by the
block grant of NCPGR (provided by DBT) to S. C., and partly by
USDA grant no. 9511747 to N. Wei. V. Y and S.K. are recipients of
CSIR fellowships, Government of India.
 
Date 2013-10-21T06:53:42Z
2013-10-21T06:53:42Z
2002
21 May 2002
 
Type Article
 
Identifier Plant Journal, 31(6): 741-753
http://hdl.handle.net/123456789/30
 
Language en
 
Publisher Wiley-Blackwell