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Leader RNA binding ability of chandipura virus P protein is regulated by its phosphorylation status: A possible role in genome transcription-replication switch

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Title Leader RNA binding ability of chandipura virus P protein is regulated by its phosphorylation status: A possible role in genome transcription-replication switch
 
Creator Basak, Soumen
Raha, Tamal
Chattopadhyay, Debasis
Majumder, Amitabha
Shaila, M.S.
Chattopadhyay, D.J.
 
Subject Leader RNA
Phosphoprotein
Transcription
Replication
Chandipura virus
 
Description The molecular events associated with the transcriptive and replicative cycle of negative-stranded RNA viruses are still an enigma. We
took Chandipura virus, a member of the Rhabdoviridae family, as our model system to demonstrate that Phosphoprotein P, besides
Nucleocapsid protein N, also acts as a leader RNA-binding protein in its unphosphorylated form, whereas CKII-mediated phosphorylation
totally abrogates its RNA-binding ability. However, interaction between P protein and leader RNA can be distinguished from N-mediated
encapsidation of viral sequences. Furthermore, P protein bound to leader chain can successively recruit N protein on RNA while itself being
replaced. We also observed that the accumulation of phosphorylation null mutant of P protein in cells results in enhanced genome RNA
replication with concurrent increase in the viral yield. All these results led us to propose a model explaining viral transcription-replication
switch where Phosphoprotein P acts as a modulator of genome transcription and replication by its ability to bind to the nascent leader RNA
in its unphosphorylated form, promoting read-through of the transcription termination signals and initiating nucleocapsid assembly on the
nascent RNA chain.
This work was supported by
DST, Govt. of India.
 
Date 2013-10-22T06:07:21Z
2013-10-22T06:07:21Z
2003
29 Oct 2002
 
Type Article
 
Identifier Virology, 307: 372-385
http://hdl.handle.net/123456789/31
 
Language en
 
Publisher Elsevier B.V.