Record Details

Light mediated regulation defines a minimal promoter region of TOP2

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Light mediated regulation defines a minimal promoter region of TOP2
 
Creator Hettiarachchi, G. H. C. M.
Yadav, Vandana
Reddy, M. K.
Chattopadhyay, Sudip
Sopory, Sudhir K.
 
Subject Light-mediated regulation
TOP2
 
Description Light signaling has been demonstrated to be an
important factor for plant growth and development;
however, its role in the regulation of DNA replication
and cell cycle has just started to be unraveled. In
this work, we have demonstrated that the TOP2
promoter of Pisum sativum (pea) is activated by a
broad spectrum of light including far-red light (FR),
red light (RL) and blue light (BL). Deletion analyses
of the TOP2 promoter in transformed plants,
Arabidopsis thaliana and Nicotiana tobaccum
(tobacco), de®ne a minimal promoter region that is
induced by RL, FR and BL, and is essential and suficient for light-mediated activation. The minimal
promoter of TOP2 follows the phytochromemediated low- ̄uence response similar to complex
light regulated promoters. DNA±protein interaction
studies reveal the presence of a DNA binding
activity speci®c to a 106 bp region of the minimal
promoter that is crucial for light-mediated activation. These results altogether indicate a direct
involvement of light signaling in the regulation of
expression of TOP2, one of the components of the
DNA replication/cell cycle machinery.
This work was
supported by the block grant of NCPGR and a DBT grant to
S.C., and internal grant of ICGEB to S.K.S.
 
Date 2013-10-22T06:17:49Z
2013-10-22T06:17:49Z
2003
30 July 2003
 
Type Article
 
Identifier Nucl. Acids Res., 31(18): 5256-5265
http://hdl.handle.net/123456789/32
 
Language en
 
Publisher Oxford University Press