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Isolation and characterization of sequence-tagged microsatellite sites markers in chickpea (Cicer arietinum L.)

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Title Isolation and characterization of sequence-tagged microsatellite sites markers in chickpea (Cicer arietinum L.)
 
Creator Sethy, Niroj K.
Shokeen, Bhumika
Bhatia, Sabhyata
 
Subject Chickpea
Cicer arietinum
microsatellites
sequence-tagged microsatellite sites
polymorphism
cross-species amplification
 
Description In this study we report the isolation of microsatellite sequences and their conversion
to sequence-tagged microsatellite sites (STMS) markers in chickpea (Cicer arietinum L.).
Thirteen putative recombinants isolated from a chickpea genomic library were sequenced,
and used to design 10 STMS primer pairs. These were utilized to analyse the genetic
polymorphism in 15 C. arietinum varieties and two wild varieties, C. echinospermum and
C. reticulatum. All the primer pairs amplified polymorphic loci ranging from four to seven
alleles per locus. The observed heterozygosity ranged from 0 to 0.6667. Most of the STMS
markers also amplified corresponding loci in the wild relatives suggesting conservation
of these markers in the genus. Hence, these polymorphic markers will be useful for the
evaluation of genetic diversity and molecular mapping in chickpea.
The authors thankfully acknowledge NCPGR, an autonomous
institute of the Department of Biotechnology, Government of
India, for supporting this work.
 
Date 2013-10-22T06:44:46Z
2013-10-22T06:44:46Z
2003
6 May 2003
 
Type Article
 
Identifier Mol. Ecology Notes, 3: 428-430
http://hdl.handle.net/123456789/34
 
Language en
 
Publisher Wiley-Blackwell