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Growth and terpenoid indole alkaloid production in Catharanthus roseus hairy root clones in relation to left- and right-termini-linked Ri T-DNA gene integration

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Title Growth and terpenoid indole alkaloid production in Catharanthus roseus hairy root clones in relation to left- and right-termini-linked Ri T-DNA gene integration
 
Creator Batra, Jyoti
Dutta, Ajaswrata
Singh, Digvijay
Kumar, Sushil
Sen, Jayanti
 
Subject Hairy root culture
Catharanthus roseus
Ri T-DNA genes
Morphological evaluation
Alkaloid production
 
Description Hairy root cultures of Catharanthus roseus var.
Prabal were established by infecting the leaves with Agrobacterium rhizogenes agropine-type A4 strain. Two hundred and fifty independent root clones were evaluated for
growth, morphology, number of integration of Ri T-DNA
genes and alkaloid contents. On the basis of growth pat-
tern, type of branching and number of lateral roots we
were able to separate the hairy root clones into four
categories. However based on the integration of the
Ri TL-DNA and TR-DNA genes, there were only three
different categories of independent hairy root clones–C1
(rolA&B+/ags+), C2 (rolA&B-/ags+) and C3 (rolA&B+/ags ). Southern hybridization analysis revealed both
single and multiple copies of T-DNA integration in the
root clones. The accumulation of considerable amounts
of the root-specific alkaloids ajmalicine and serpentine was observed in the presence of both the TL-DNA
and TR-DNA genes (C1) and the TL-DNA gene (C3)
alone. Two rolA&B but ags+ clones (C2) accumulated
much less or only very negligible amounts of ajmalicine.
The possible role of the TL-DNA and TR-DNA genes on
growth and alkaloid accumulation in these root clones is
discussed
We sincerely thank the Department of Bio-
technology, Government of India, for financial support.
 
Date 2013-10-29T04:41:50Z
2013-10-29T04:41:50Z
2004
28 April 2004
 
Type Article
 
Identifier Plant Cell Reports, 23: 148-154
http://hdl.handle.net/123456789/35
 
Language en
 
Publisher Springer