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Expression of terpenoid indole alkaloid biosynthetic pathway genes corresponds to accumulation of related alkaloids in Catharanthus roseus (L.) G. Don

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Title Expression of terpenoid indole alkaloid biosynthetic pathway genes corresponds to accumulation of related alkaloids in Catharanthus roseus (L.) G. Don
 
Creator Dutta, Ajaswrata
Batra, Jyoti
Pandey-Rai, Sashi
Singh, Digvijay
Kumar, Sushil
Sen, Jayanti
 
Subject Catharanthus
Gene expression
Genetic resource
Morphological and biochemical variations
Terpenoid indole alkaloid pathway
Alkaloid contents
 
Description Madagascar periwinkle, Catharanthus roseus
(L.) G. Don, a medicinally important plant, produces
anticancer dimeric alkaloids, vinblastine and vincristine,
in the leaves and accumulates antihypertensive alkaloids, ajmalicine and serpentine, in the roots. This plant
grows wild in distant tropical and sub-tropical geographical locations with different agro-climates and
shows wide variations in morphological and alkaloid
yield-related traits. In order to understand the correlation between the expression of terpenoid indole alkaloid
(TIA) pathway genes and accumulation of related
alkaloids, six different genetic resources of C. roseus,
including the medicinal cultivars Nirmal, Prabal, Dhawal, the mutants gsr-3 and gsr-6, and one horticultural
variety, Pacifica blush, were studied. The expression
profiles of one early and two late TIA biosynthetic
pathway genes, namely, strictosidine synthase, desacetoxyvindoline 4-hydroxylase and deacetyl vindoline 4-O-acetyl transferase were analyzed in these plants. A positive correlation between transcript abundance and
accumulation of related alkaloids was observed in the
different genetic resources. The potential of these TIA
biosynthetic pathway genes for use in screening of high-yielding C. roseus germplasm has been discussed.
Grateful thanks are due to the Department of
Biotechnology, Government of India, for their financial support to
this project.
 
Date 2013-10-30T09:47:37Z
2013-10-30T09:47:37Z
2005
6 July 2004
 
Type Article
 
Identifier Planta, 220: 376-383
http://hdl.handle.net/123456789/40
 
Language en
 
Publisher Springer