Record Details


Field	Value

Title	Development of STMS markers from the medicinal plant Madagascar periwinkle (Catharanthus roseus L.) G. Don

Creator	Shokeen, Bhumika Sethy, Niroj K. Choudhary, Shalu Bhatia, Sabhyata

Subject	Catharanthus roseus degenerate primers microsatellites STMS

Description	We report the isolation and characterization of the first set of sequence-tagged microsatellites sites (STMS) markers in Catharanthus roseus, a plant with a vast range of medicinal uses. The microsatellite loci were cloned from an enriched library constructed using degenerate primers. Based on the microsatellite motifs, seven STMS primer pairs were designed. They were used to amplify 32 accessions of C. roseus and one accession of Catharanthus trichophyllus. The primers amplified an average of 3.86 alleles per locus. The observed heterozygosity ranged from 0.2903 to 0.9688 with an average of 0.7511. The STMS markers of C. roseus also amplified corresponding loci in a related species (C. trichophyllus) suggesting conservation of the loci across the genus. These markers will prove useful for genetic diversity analysis and linkage map construction in C. roseus. The authors thankfully acknowledge NCPGR, an autonomous institute of Department of Biotechnology, Government of India, for providing financial support to this work.

Date	2013-10-31T08:29:44Z 2013-10-31T08:29:44Z 2005 13 May 2005

Type	Article

Identifier	Mol. Ecology Notes, 5: 818-820 http://hdl.handle.net/123456789/45

Language	en

Publisher	Wiley-Blackwell

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