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Development of sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.)

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Title Development of sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.)
 
Creator Choudhary, Shalu
Sethy, Niroj K.
Shokeen, Bhumika
Bhatia, Sabhyata
 
Subject chickpea
Cicer reticulatum
mapping
microsatellites
STMS
 
Description Microsatellite loci were identified from chickpea (Cicer arietinum L.), the third most important
grain legume crop in the world. A total of 13 sequence-tagged microsatellite markers were
developed using two different approaches: (i) amplification using degenerate primers and
(ii) cloning of intersimple sequence repeat (ISSR)-amplified fragments. Thirty-five chickpea
accessions were analysed, which resulted in a total of 30 alleles at the 13 loci. The observed
heterozygosity ranged from 0.1143 to 0.4571 with an average of 0.2284. The cross-species
transferability of the sequence-tagged microsatellite site (STMS) markers was checked in
Cicer reticulatum, the wild annual progenitor of chickpea. These microsatellite markers will be useful for assessing the genetic diversity patterns within chickpea as well as aid in
construction of intra- and interspecific genetic linkage maps.
Financial assistance for this work was provided by Department of Biotechnology, Government of India (BT/PR3518/AGR/02/179/
2002) and National Centre for Plant Genome Research.
 
Date 2013-11-04T06:48:06Z
2013-11-04T06:48:06Z
2006
25 July 2005
 
Type Article
 
Identifier Mol. Ecology Notes, 6: 93-95
http://hdl.handle.net/123456789/48
 
Language en
 
Publisher Wiley-Blackwell