Record Details

Expression of CAP2, an AP2-family transcription factor from chickpea enhances growth and tolerance to dehydration and salt tress in transgenic tobacco

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Expression of CAP2, an AP2-family transcription factor from chickpea enhances growth and tolerance to dehydration and salt tress in transgenic tobacco
 
Creator Shukla, Rakesh K.
Raha, Sumita
Tripathi, Vineeta
Chattopadhyay, Debasis
 
Subject CAP2
chickpea
Tobacco
PETALA2-Family Transcription Factor
Transgenic Tobacco
Dehydration
Salt Stress
 
Description The APETALA2 (AP2) domain defines a large family of DNA-binding proteins that play important roles in plant morphology,
development, and stress response. We describe isolation and characterization of a gene (CAP2) from chickpea (Cicer arietinum)
encoding a novel AP2-family transcription factor. Recombinant CAP2 protein bound specifically to C-repeat/dehydration-
responsive element in gel-shift assay and transactivated reporter genes in yeast (Saccharomyces cerevisiae) one-hybrid assay.
CAP2 appeared to be a single/low copy intronless gene, and the protein product localized in the nucleus. Transcript level of
CAP2 increased by dehydration and by treatment with sodium chloride, abscisic acid, and auxin, but not by treatment with
low temperature, salicylic acid, and jasmonic acid. The 35S promoter-driven expression of CAP2 in tobacco (Nicotiana tabacum)
caused drastic increase in the leaf cell size, and, thereby, in leaf surface area and number of lateral roots. Transgenic plants
demonstrated more tolerance to dehydration and salt stress than the wild-type plants. Transgenic plants expressed higher
steady-state transcript levels of abiotic stress-response genes NtERD10B and NtERD10C and auxin-response genes IAA4.2 and
IAA2.5. Taken together, our results indicated a mutual interrelation between plant growth-development and abiotic stress-
response pathways and a probable involvement of CAP2 in both the signaling pathways.
This work was supported by the National Centre for Plant
Genome Research and a grant from the Department of Biotechnology, Government of India (DBT). R.K.S. and V.T. acknowledge
Council for Scientific and Industrial Research and S.R. acknowledges
DBT for fellowships.
 
Date 2013-11-05T04:31:38Z
2013-11-05T04:31:38Z
2006
11 July 2006
 
Type Article
 
Identifier Plant Physiol., 142: 113-123
http://hdl.handle.net/123456789/60
 
Language en
 
Publisher American Society of Plant Biologists