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Identification of sex in Simmondsia chinensis (Jojoba) using RAPD markers

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Title Identification of sex in Simmondsia chinensis (Jojoba) using RAPD markers
 
Creator Agrawal, Veena
Sharma, Kuldeep
Gupta, Sarika
Kumar, Ravindra
Prasad, Manoj
 
Subject Simmondsia chinensis
Dioecious
RAPD markers
Bulk segregant analysis
 
Description Simmondsia chinensis (Link) Schneider, a
multipurpose dioecious shrub of arid zones, has emerged as
a cash crop. It is being cultivated for its seeds which store
liquid wax whose properties are similar to spermaceti
(Sperm whale oil), a substitute for petro products and
precious high-priced lubricants. Jojoba is a slow-growing
desert shrub having a male biased (5:1; male:female ratio)
population. Since there is no method available to determine
the sex at the seedling stage, current investigations have
been carried out to generate a sex-specific random amplified polymorphic DNA (RAPD) marker in jojoba which is
based on the PCR amplification of random locations in the
genome of plant. Of the 72 primers tested, only one random
decamer primer, OPG-5, produced a unique *1,400 base
pairs fragment in male DNA. To validate this observation,
this primer was re-tested with the individuals of male and
female samples of four cultivars. The unique *1,400 bp fragment was present in male individuals of all the four
cultivars and completely absent in respective female individuals tested. To the best of our knowledge, this is the first
report to ascertain the sex of jojoba plants at an early stage
of development of the taxon.
V.A. is grateful to the University Grants Commission and University of Delhi, New Delhi for financial assistance.
Facilities provided by the Director, National Institute for Plant Genome Research, New Delhi are gratefully acknowledged. We are also
thankful to the AJORP (Association of the Rajasthan Jojoba Plantation and Research Project) for research material. K.D. is indebted to
CSIR, New Delhi for awarding SRF.
 
Date 2013-11-05T04:51:45Z
2013-11-05T04:51:45Z
2007
4 September 2007
 
Type Article
 
Identifier Plant Biotechnology Reports, 1: 207-210
http://hdl.handle.net/123456789/61
 
Language en
 
Publisher Springer