Record Details

Comparative proteomics analysis of differentially expressed proteins in chickpea extracellular matrix during dehydration stress

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Comparative proteomics analysis of differentially expressed proteins in chickpea extracellular matrix during dehydration stress
 
Creator Bhushan, Deepti
Pandey, Aarti
Choudhary, Mani Kant
Datta, Asis
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Dehydration Stress
Chickpea
Chickpea Extracellular Matrix
Proteomics Analysis
 
Description Water deficit or dehydration is the most crucial environmental factor that limits crop productivity and influences
geographical distribution of many crop plants. It is suggested that dehydration-responsive changes in expression of proteins may lead to cellular adaptation against
water deficit conditions. Most of the earlier understanding
of dehydration-responsive cellular adaptation has evolved
from transcriptome analyses. By contrast, comparative
analysis of dehydration-responsive proteins, particularly
proteins in the subcellular fraction, is limiting. In plants,
cell wall or extracellular matrix (ECM) serves as the repository for most of the components of the cell signaling
process and acts as a frontline defense. Thus, we have
initiated a proteomics approach to identify dehydration-
responsive ECM proteins in a food legume, chickpea.
Several commercial chickpea varieties were screened for
the status of dehydration tolerance using different physiological and biochemical indexes. Dehydration-responsive temporal changes of ECM proteins in JG-62, a relatively tolerant variety, revealed 186 proteins with variance
at a 95% significance level statistically. The comparative
proteomics analysis led to the identification of 134 differentially expressed proteins that include predicted and
novel dehydration-responsive proteins. This study, for the
first time, demonstrates that over a hundred ECM proteins, presumably involved in a variety of cellular functions, viz. cell wall modification, signal transduction, metabolism, and cell defense and rescue, impinge on the
molecular mechanism of dehydration tolerance in
plants.
This work was supported by grants from the Department of Biotechnology, Ministry of Science and Technology (to N. C.) and by
predoctoral fellowships (to D. B., A. P., and M. K. C.) from the Council
of Scientific and Industrial Research, Government of India.
 
Date 2013-11-05T05:27:54Z
2013-11-05T05:27:54Z
2007
1 August 2007
 
Type Article
 
Identifier Mol. Cell. Proteomics, 6: 1868 -1884
http://hdl.handle.net/123456789/63
 
Language en
 
Publisher The American Society for Biochemistry and Molecular Biology, Inc.