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Transcript profiling of terpenoid indole alkaloid pathway genes and regulators reveals strong expression of repressors in Catharanthus roseus cell cultures

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Title Transcript profiling of terpenoid indole alkaloid pathway genes and regulators reveals strong expression of repressors in Catharanthus roseus cell cultures
 
Creator Dutta, Ajaswrata
Singh, Digvijay
Kumar, Sushil
Sen, Jayanti
 
Subject Catharanthus roseus
Primary and TIA pathway transcripts
Rotation cell cultures
TIA pathway repressors
TIAs
 
Description The understanding of the complexities and
molecular events regulating genes and the activators
involved in terpenoid indole alkaloid (TIA) metabolism is known to a certain extent in cell cultures of an
important TIA yielding plant, Catharanthus roseus,
though it is not yet complete. Recently, the repressors
of early TIA pathway genes have also been identified.
However, their roles in the regulation of TIA pathway
in C. roseus cell cultures remains yet unknown. We
have made a comparative profiling of genes catalyzing
the important steps of 2-C methyl-D-erythritol-4-
phosphate (MEP), shikimate and TIA biosynthetic
pathways, their activator and repressors using macro-
array, semiquantitative RT-PCR and northern analyses
in a rotation culture system of C. roseus comprising
differentiated and proliferated cells. Our results demonstrate that TIA biosynthetic pathway genes and their
activators show variable expression pattern, which was
correlated with the changes in the cellular conditions in
these systems. Under similar conditions, TIA pathway
repressors show strong and consistent expression. The
role of repressors in the complex regulation of the TIA pathway in C. roseus cell cultures is discussed. The
results were supported by HPLC data, which demonstrated that the molecular program of cellular differentiation is intimately linked with TIA pathway gene
expression and TIA production in C. roseus cell cultures.
 
Date 2013-11-05T05:52:58Z
2013-11-05T05:52:58Z
2007
2 January 2007
 
Type Article
 
Identifier Plant Cell Reports, 26: 907-915
http://hdl.handle.net/123456789/66
 
Language en
 
Publisher Springer