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Construction of genetic linkage map of the medicinal and ornamental plant Catharanthus roseus

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Title Construction of genetic linkage map of the medicinal and ornamental plant Catharanthus roseus
 
Creator Gupta, Sarika
Pandey-Rai, Sashi
Srivastava, Suchi
Naithani, Subhas Chandra
Prasad, Manoj
Kumar, Sushil
 
Subject Catharanthus roseus
ornamental plant
medicinal plant
genetic linkage map
 
Description An integrated genetic linkage map of the medicinal and ornamental plant Catharanthus roseus, based on different types of
molecular and morphological markers was constructed, using a F2 population of 144 plants. The map defines 14 linkage
groups (LGs) and consists of 131 marker loci, including 125 molecular DNA markers (76 RAPD, 3 RAPD combinations; 7
ISSR; 2 EST-SSR from Medicago truncatula and 37 other PCR based DNA markers), selected from a total of 472 primers
or primer pairs, and six morphological markers (stem pigmentation, leaf lamina pigmentation and shape, leaf petiole and
pod size, and petal colour). The total map length is 1131.9 cM (centiMorgans), giving an average map length and distance
between two markers equal to 80.9 cM and 8.6 cM, respectively. The morphological markers/genes were found linked with
nearest molecular or morphological markers at distances varying from 0.7 to 11.4 cM. Linkage was observed between the
morphological markers concerned with lamina shape and petiole size of leaf on LG1 and leaf, stem and petiole pigmentation
and pod size on LG8. This is the first genetic linkage map of C. roseus.
Grateful thanks are due to the Department of
Biotechnology, Government of India and Indian National Science Academy for providing financial support.
 
Date 2013-11-05T09:54:07Z
2013-11-05T09:54:07Z
2007
October 2007
 
Type Article
 
Identifier J. Genet., 86(3): 259-268
http://hdl.handle.net/123456789/68
 
Language en
 
Publisher Indian Academy of Sciences