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Isolation and characterization of microsatellite markers for analysis of molecular variation in the medicinal plant Madagascar periwinkle (Catharanthus roseus (L.) G. Don.)

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Title Isolation and characterization of microsatellite markers for analysis of molecular variation in the medicinal plant Madagascar periwinkle (Catharanthus roseus (L.) G. Don.)
 
Creator Shokeen, Bhumika
Sethy, Niroj Kumar
Kumar, Sushil
Bhatia, Sabhyata
 
Subject Catharanthus roseus
Microsatellites
Genetic diversity
Madagascar periwinkle
STMS
 
Description Catharanthus roseus (L.) G. Don, commonly known as Madagascar periwinkle, is a plant of great medicinal value. In this study microsatellite
markers were developed and utilized for analysis of genetic diversity in C. roseus. In order to isolate microsatellite sequences, two small insert
genomic libraries of C. roseus cv. Nirmal were constructed and screened with CA, CT, GC and GCG oligonucleotide repeats. Sixty-five
microsatellite motifs were identified, from which 38 functional STMS primer pairs were designed and validated. Out of these, 24 STMS markers
were used to evaluate the genetic polymorphism in 37 genotypes, which comprised of 32 accessions of C. roseus, a single accession each of two
related species (C. trichophyllus and C. pusillus) and one accession each of three related genera (Vinca minor, Thevetia peruviana and Nerium
indicum). The 24 STMS markers detected 26 loci with two markers amplifying more than one locus. A total of 124 alleles were amplified in the 37
genotypes ranging from 2 to 10 alleles with an average of 4.76 alleles per locus. The high average expected heterozygosity (H) value of 0.56 and
observed heterozygosity (H) value of 0.52 established the efficiency of the STMS markers for discriminating the C. roseus genotypes. Nei and Li’s
similarity coefficients were calculated and a UPGMA-based dendrogram was constructed which clearly distinguished all genotypes except two
pairs. Sequence analysis of the length variant alleles at three STMS loci revealed that the variation in the copy number of repeat motifs was the
major source of length polymorphism within C. roseus. However, isolated point mutations and indels in the microsatellite flanking regions (MFRs)
of homologus loci from other species also contributed to size homoplasy and allelic size variation. This study is the first report of microsatellite
development and utilization in C. roseus, providing significant insights into its genome structure and organization.
 
Date 2013-11-06T05:50:42Z
2013-11-06T05:50:42Z
2007
17 October 2006
 
Type Article
 
Identifier Plant Science 172: 441-451
http://hdl.handle.net/123456789/76
 
Language en
 
Publisher Elsevier B.V.