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Comparative proteomics of tuber induction, development and maturation reveal the complexity of tuberization process in potato (Solanum tuberosum L.)

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Title Comparative proteomics of tuber induction, development and maturation reveal the complexity of tuberization process in potato (Solanum tuberosum L.)
 
Creator Agrawal, Lalit
Chakraborty, Subhra
Jaiswal, Dinesh Kumar
Gupta, Sonika
Datta, Asis
Chakraborty, Niranjan
 
Subject tuber development
storage proteins
differential display
mass spectrometry
cluster analysis
ROS pathway
 
Description Tuberization in potato ( Solanum tuberosum L.) is a developmental process that serves a double function, as a storage organ and as a vegetative propagation system. It is a multistep, complex process and the underlying mechanisms governing these overlapping steps are not fully understood. To understand the molecular basis of tuberization in potato, a comparative proteomic approach has been applied to monitor differentially expressed proteins at different development stages using two-dimensional gel electrophoresis (2-DE). The differentially displayed proteomes revealed 219 protein spots that change their intensities more than 2.5-fold. The LC-ES-MS/MS analyses led to the identification of 97 differentially regulated proteins that include predicted and novel tuber-specific proteins. Nonhierarchical clustering revealed coexpression patterns of functionally similar proteins. The expression of reactive oxygen species catabolizing enzymes, viz., superoxide dismutase, ascorbate peroxidase and catalase, were induced by more than 2-fold indicating their possible role during the developmental transition from stolons into tubers. We demonstrate that nearly 100 proteins, some presumably associated with tuber cell differentiation, regulate diverse functions like protein biogenesis and storage, bioenergy and metabolism, and cell defense and rescue impinge on the complexity of tuber development in potato.
This work was supported by grants
from the Council of Scientific and Industrial Research(CSIR), Ministry of Science and Technology, India.
 
Date 2013-11-06T10:22:40Z
2013-11-06T10:22:40Z
2008
July 2008
 
Type Article
 
Identifier J. Proteome Res., 7(9): 3803-3817
http://hdl.handle.net/123456789/79
 
Language en
 
Publisher American Chemical Society