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Cloning and characterization of a novel LpWRKY1 transcription factor in tomato

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Title Cloning and characterization of a novel LpWRKY1 transcription factor in tomato
 
Creator Hofmann, Markus Georg
Sinha, Alok Krishna
Proels, Reinhard Korbinian
Roitsch, Thomas
 
Subject WRKY
Transcription factor
Phosphorylation
Tomato
Stress
Elicitor
 
Description The initiation of defence responses in plants is accompanied by fundamental changes in gene expression: the expression of pathogenesis-
related genes is co-ordinately regulated with metabolic changes such as down regulation of photosynthesis and induction of sink metabolism.
To identify candidate regulators of this co-ordinated regulatory mechanism, the role of WRKY transcription factors in the initiation of defence
response was analysed in tomato. A WRKY-type transcription factor (LpWRKY1) from tomato was cloned by a reverse Northern approach. The
corresponding mRNA is rapidly and transiently induced after challenging the cells with an elicitor-preparation derived from the wilt inducing
fungus Fusarium oxysporum lycopersici (E-FOL) and the fungal elicitor chitosan, whereas the endogenous signals systemin and salicylic acid are
inactive. Inhibition of protein biosynthesis by cycloheximide results in sustained induction of mRNA for LpWRKY1. In contrast, the transient
induction of the gene encoding LpWRKY1 in response to elicitation by E-FOL is inhibited by the protein-kinase inhibitor staurosporine and may
be mimicked by the phosphatase inhibitors endothall and cantharidine indicating the involvement of protein phosphorylation in the regulation of
WRKY-type transcription factors. Direct proof of this postranslational modification of LpWRKY1 was obtained by demonstrating in-gel kinase
assays using recombinant LpWRKY1 as substrate. A 44 kDa and a 67 kDa protein kinase were shown to be transiently activated to phosphorylate LpWRKY1 protein in response to elicitation with E-FOL.
Financial support by the Deutsche Forschungsgemeinschaft
(DFG) to T.R. (Ro4-1), by the Alexander von Humboldt foun-
dation to A.K.S., and by the Studienstiftung des Deutschen
Volkes to M.H. is acknowledged.
 
Date 2013-11-07T06:14:14Z
2013-11-07T06:14:14Z
2008
February 2008
 
Type Article
 
Identifier Plant Physiology and Biochemistry, 46(5-6): 533-540
http://hdl.handle.net/123456789/85
 
Language en
 
Publisher Elsevier B.V.