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Two divergent genes encoding L-myo-inositol 1-phosphate synthase1 (CaMIPS1) and 2 (CaMIPS2) are differentially expressed in chickpea

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Title Two divergent genes encoding L-myo-inositol 1-phosphate synthase1 (CaMIPS1) and 2 (CaMIPS2) are differentially expressed in chickpea
 
Creator Kaur, Harmeet
Shukla, Rakesh Kumar
Yadav, Gitanjali
Chattopadhyay, Debasis
Majee, Manoj
 
Subject differential expression
environmental stress
functional complementation
gene duplication
 
Description L-myo-inositol 1-phosphate synthase (MIPS; EC5.5.1.4)
catalyses the rate-limiting step in inositol biosynthetic
pathway, and is extremely widespread in living organisms
including plants. Several plants possess multiple copies of
MIPS gene(s) indicating a possibility of differential expression of each gene to perform distinct physiological functions. To explore this, two MIPS genes (CaMIPS1 and
CaMIPS2) were isolated from a drought-tolerant plant
chickpea. Both genes are extremely divergent in respect to
their introns, at the same time retaining 85% identity to
their exons and functionally complementing inositol auxotroph Schizosaccharomyces pombe. Expression analysis
showed both genes were expressed in all organs except
seed, where only CaMIPS2 transcript was detected. Under
environmental stresses, only CaMIPS2 was induced
whereas CaMIPS1 expression remained same, which could
be explained by the divergence of their 5' upstream regulatory sequences. Remarkably, both gene products exhibited similar biochemical characteristics; however, CaMIPS2
retained higher activity than CaMIPS1 at a high temperature and salt concentration. Furthermore, functional
expression of CaMIPS2 in S. pombe results better growth
response than CaMIPS1 under stress environment. Taken
together, our results suggest that CaMIPS1 and CaMIPS2
are differentially expressed in chickpea to play discrete
though overlapping roles in plant; however CaMIPS2 is
likely to be evolved through gene duplication to function
under environmental stresses.
We would like to acknowledge the financial support from
National Institute for Plant Genome Research, Department
of Biotechnology, Government of India
 
Date 2013-11-07T09:56:58Z
2013-11-07T09:56:58Z
2008
7 August 2008
 
Type Article
 
Identifier Plant Cell and Environment, 31: 1701-1716
http://hdl.handle.net/123456789/89
 
Language en
 
Publisher Wiley-Blackwell