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Proteomics approach to identify dehydration responsive nuclear proteins from chickpea (Cicer arietinum L.)

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Title Proteomics approach to identify dehydration responsive nuclear proteins from chickpea (Cicer arietinum L.)
 
Creator Pandey, Aarti
Chakraborty, Subhra
Datta, Asis
Chakraborty, Niranjan
 
Subject chickpea
Cicer arietinum L.
Proteomics
Dehydration Responsive
Nuclear Proteins
 
Description Dehydration or water-deficit is one of the most important
environmental stress factors that greatly influences plant
growth and development and limits crop productivity.
Plants respond and adapt to such stress by altering their
cellular metabolism and activating various defense machineries. Mechanisms that operate signal perception,
transduction, and downstream regulatory events provide
valuable information about the underlying pathways involved in environmental stress responses. The nuclear
proteins constitute a highly organized, complex network
that plays diverse roles during cellular development and
other physiological processes. To gain a better understanding of dehydration response in plants, we have developed a comparative nuclear proteome in a food legume, chickpea (Cicer arietinum L.). Three-week-old
chickpea seedlings were subjected to progressive dehydration by withdrawing water and the changes in the nuclear proteome were examined using two-dimensional gel
electrophoresis. Approximately 205 protein spots were
found to be differentially regulated under dehydration.
Mass spectrometry analysis allowed the identification of
147 differentially expressed proteins, presumably involved
in a variety of functions including gene transcription and
replication, molecular chaperones, cell signaling, and
chromatin remodeling. The dehydration responsive nuclear proteome of chickpea revealed a coordinated response, which involves both the regulatory as well as the
functional proteins. This study, for the first time, provides
an insight into the complex metabolic network operating
in the nucleus during dehydration.
This research work was supported by grants from the Department
of Biotechnology, Ministry of Science and Technology, India
 
Date 2013-11-11T06:49:57Z
2013-11-11T06:49:57Z
2008
October 2007
 
Type Article
 
Identifier Mol. Cell. Proteomics, 7(1): 88-107
http://hdl.handle.net/123456789/96
 
Language en
 
Publisher The American Society for Biochemistry and Molecular Biology, Inc.