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ISSR marker assisted selection of male and female plants in a promising dioecious crop-Jojoba (Simmondsia chinensis)

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Title ISSR marker assisted selection of male and female plants in a promising dioecious crop-Jojoba (Simmondsia chinensis)
 
Creator Sharma, Kuldeep
Agrawal, Veena
Gupta, Sarika
Kumar, Ravindra
Prasad, Manoj
 
Subject Dioecious
ISSR marker
Simmondsia chinensis
 
Description Simmondsia chinensis (Link) Schneider, a
multipurpose and monogeneric dioecious shrub from arid
zones, has emerged as a cash crop all over the globe. Its
seed propagation poses severe problems due to its
male-biased population: the male:female ratio is 5:1.
Investigations have been carried out to generate a
sex-specific Inter-simple sequence repeat (ISSR) marker
for the early detection of male and female plants. Of the 42
primers analysed with a bulk sample of pooled male DNA
and a bulk sample of pooled female DNA, only one primer,
UBC-807, produced a unique ~1,200 base-pair fragment
in the male DNA. To validate this observation, this primer
was re-tested with individual male and female samples
from eight cultivars. A similar unique ~1,200 bp fragment
was present in the male individuals of all eight cultivars
and completely absent in the female individuals tested.
This is the first report of the use of ISSR markers to
ascertain sex in physiologically mature S. chinensis plants.
Veena Agrawal is grateful to the University
Grants Commission and the University of Delhi for financial assistance. Facilities provided by the Head of the Department of Botany at
the University of Delhi and the Director of the National Institute for
Plant Genome Research New Delhi are gratefully acknowledged. We
also thank the AJORP (Association of the Rajasthan Jojoba Plantation
and Research Project) for research material. Kuldeep Sharma is
indebted to CSIR New Delhi for awarding SRF.
 
Date 2013-11-12T06:11:30Z
2013-11-12T06:11:30Z
2008
10 August 2008
 
Type Article
 
Identifier Plant Biotechnology Reports, 2: 239-243
http://hdl.handle.net/123456789/100
 
Language en
 
Publisher Springer